Comparative Pathology and Genomics Shared Resource
Comparative Pathology and Genomics Shared Resource
The Comparative Pathology and Genomics Shared Resource (CPGSR) provides outstanding support for biomedical research to assess and improve animal models of disease for academic and industry partners within Massachusetts and beyond. Our expertise in pathology and genomics allows us to perform detailed characterization of animal models across species and research areas via histopathology, DNA and RNA interrogation, and spatial assessment assays.
Services
Pathology
Study design consultation
Animal model validation by board-certified veterinary pathologists
Histopathology assessment and interpretation
Pathology image capture, annotation, and figure preparation for manuscripts
Toxicity assessment test articles
Histology
Trimming fixed tissue to paraffin blocks
Processing frozen tissue to OCT blocks
Frozen sectioning
Sectioning paraffin blocks to unstained slides
Slide staining
Routine H&E (hematoxylin and eosin)
Special stains, including acid-fast, trichrome, and silver
Immunohistochemistry and immunofluorescence
RNAScope in situ hybridization
Whole slide scanning (20X and 40X, brightfield and fluorescent)
Semi-quantitative scoring and Digital Quantitative Image Analysis
Tissue classification
IHC/special stain area quantification
IHC single cell detection and quantification
IF multiplex/highplex image analysis
Genomics
10X 3’ Kit (Live cell, nuclei, and FFPE options)
Nanostring nCounter Panels
Sequencing with NextSeq 2000
Library Preparations
Illumina Stranded Total RNA
KAPA HyperPrep
NEB emSEQ
Custom Requests
Nucleic Acid Extraction
Apostle Cell-free DNA Extraction
Apostle Cell-free RNA Extraction
QIAGEN DNeasy (Blood and Tissue)
NEB Monarch Genomic DNA Purification Kit
NEB Monarch Total RNA Mini-Prep Kit
QIAGEN RNeasy Mini-Kit
DNA GenoTek PERFORMAgene DNA Extraction (Animals)
Quality Control
KAPA Library Quantification
Quant-iT PicoGreen dsDNA Assay
Quant-iT RiboGreen RNA Assay
Qubit Flex
Agilent TapeStation 4200
Spatial transcriptomics
NanoString GeoMx Digital Spatial Profiler
NanoString CosMx Spatial Molecular Imager
LiCor Odyssey M
Pippin Prep
Incucyte
Cell sorter (Sony SH800)
Flow cytometry
Applied Biosciences Absolute Q Digital PCR
10X Genomics Chromium X
10X Genomics Chromium Controller (BSL3/RBL)
Technologies/Equipment/Instruments/Resources
Histology
TissueTek Automatic H&E Stainer
Biocare IntelliPath Automatic Stainer
IHC
Leica Bond RX Automatic Stainer
IHC, IF, RNAScope
3D Histech PANNORAMIC MIDI II Digital Slide Scanner
Brightfield or fluorescent scanning
20X or 40X magnification
Genomics
NanoString nCounter Max
Illumina NextSeq 2000
10X Genomics Chromium Controller
10X Genomics Chromium X
Agilent TapeStation Automated Electrophoresis
Beckman Coulter Biomek i7 Automated Station Liquid Handling System
Incucyte
Cell sorter (Sony SH800)
Flow cytometry
Applied Biosciences Absolute Q Digital PCR
Image Analysis
Indica Labs HALO Quantitative Image Analysis Suite
Spatial transcriptomics
NanoString GeoMx Digital Spatial Profiler
NanoString CosMx Spatial Molecular Imager
Guidelines and Policies
All requests for services must be submitted on iLabs.
We are happy to accommodate quote requests for grant submissions. These requests should also be submitted via iLabs.
Work requests are processed in the order in which they are submitted. Expedite fees may apply to work requested outside of this order.
Investigators will be notified when work is completed and samples may be collected or shipped to the lab within 30 days of project completion. Long-term storage of samples will incur storage fees.
For questions about services, please email CPathGenSR@tufts.edu. This email is monitored by all staff of CPGSR.
Make a Request
CPGSR iLabs - Select “Register” in the top right corner to make an account.