Cummings School of Veterinary Medicine

Comparative Pathology and Genomics Shared Resource

The Comparative Pathology and Genomics Shared Resource (CPGSR) provides outstanding support for biomedical research to assess and improve animal models of disease for academic and industry partners within Massachusetts and beyond. Our expertise in pathology and genomics allows us to perform detailed characterization of animal models across species and research areas via histopathology, DNA and RNA interrogation, and spatial assessment assays.

Services

    • Pathology
      • Study design consultation
      •  Animal model validation by board-certified veterinary pathologists
      • Histopathology assessment and interpretation
      • Pathology image capture, annotation, and figure preparation for manuscripts
      • Toxicity assessment test articles
      • Histology
        • Trimming fixed tissue to paraffin blocks
        • Processing frozen tissue to OCT blocks
        • Frozen sectioning
        • Sectioning paraffin blocks to unstained slides
        • Slide staining
          • Routine H&E (hematoxylin and eosin) 
          • Special stains, including acid-fast, trichrome, and silver
          • Immunohistochemistry and immunofluorescence
          • RNAScope in situ hybridization 
        • Whole slide scanning (20X and 40X, brightfield and fluorescent)
        • Semi-quantitative scoring and Digital Quantitative Image Analysis 
          • Tissue classification
          • IHC/special stain area quantification
          • IHC single cell detection and quantification
          • IF multiplex/highplex image analysis
      • Genomics
        • 10X 3’ Kit (Live cell, nuclei, and FFPE options)
        • Nanostring nCounter Panels
        • Sequencing with NextSeq 2000
        • Library Preparations
          • Illumina Stranded Total RNA
          • KAPA HyperPrep
          • NEB emSEQ
          • Custom Requests
        • Nucleic Acid Extraction
          • Apostle Cell-free DNA Extraction
          • Apostle Cell-free RNA Extraction
          • QIAGEN DNeasy (Blood and Tissue)
          • NEB Monarch Genomic DNA Purification Kit
          • NEB Monarch Total RNA Mini-Prep Kit
          • QIAGEN RNeasy Mini-Kit
          • DNA GenoTek PERFORMAgene DNA Extraction (Animals)
        • Quality Control
          • KAPA Library Quantification
          • Quant-iT PicoGreen dsDNA Assay
          • Quant-iT RiboGreen RNA Assay
          • Qubit Flex
          • Agilent TapeStation 4200
      • Spatial transcriptomics
        • NanoString GeoMx Digital Spatial Profiler
        • NanoString CosMx Spatial Molecular Imager

    • LiCor Odyssey M
    • Pippin Prep
    • Incucyte
    • Cell sorter (Sony SH800)
    • Flow cytometry
    • Applied Biosciences Absolute Q Digital PCR
    • 10X Genomics Chromium X
    • 10X Genomics Chromium Controller (BSL3/RBL)

Technologies/Equipment/Instruments/Resources

    • Histology
      • TissueTek Automatic H&E Stainer
      • Biocare IntelliPath Automatic Stainer
        • IHC
      • Leica Bond RX Automatic Stainer
        • IHC, IF, RNAScope
      • 3D Histech PANNORAMIC MIDI II Digital Slide Scanner
        • Brightfield or fluorescent scanning
        • 20X or 40X magnification
    • Genomics
      • NanoString nCounter Max
      • Illumina NextSeq 2000
      • 10X Genomics Chromium Controller
      • 10X Genomics Chromium X
      • Agilent TapeStation Automated Electrophoresis
      • Beckman Coulter Biomek i7 Automated Station Liquid Handling System
      • Incucyte
      • Cell sorter (Sony SH800)
      • Flow cytometry 
      • Applied Biosciences Absolute Q Digital PCR
    • Image Analysis
      • Indica Labs HALO Quantitative Image Analysis Suite
    • Spatial transcriptomics
      • NanoString GeoMx Digital Spatial Profiler
      • NanoString CosMx Spatial Molecular Imager

Autumn Berlied standing in comparative pathobiology genomics and shared resource space

Guidelines and Policies

  • All requests for services must be submitted on iLabs.
  • We are happy to accommodate quote requests for grant submissions. These requests should also be submitted via iLabs.
  • Work requests are processed in the order in which they are submitted. Expedite fees may apply to work requested outside of this order.
  • Investigators will be notified when work is completed and samples may be collected or shipped to the lab within 30 days of project completion. Long-term storage of samples will incur storage fees.
  • For questions about services, please email CPathGenSR@tufts.edu. This email is monitored by all staff of CPGSR.
computer screen with dna imagery

Make a Request

Comparative Pathology

Co-director: Dr. Amanda Martinot, DVM MPH PhD 
Diplomate of the American College of Veterinary Pathologists (Anatomic)

Dr. Claire Lyons, DVM
Board-Eligible, American College of Veterinary Pathologists (Anatomic)

Autumn Berlied, BS
Histotechnologist

Genomics

Co-director: Dr. Heather Gardner, DVM PhD
Diplomate of the American College of Veterinary Internal Medicine (Oncology)

Alaina Ryan, BS
Senior Research Associate

News

Individual with glasses smiling and sitting at a microscope.

CVDL Through the Years

Dr. Joyce Knoll and Cheryl Stockman have led the diagnostic lab for more than three decades to serve the hospitals and clinics of Cummings School

Contact

Shipping Address:
200 Westboro Road
Peabody Room 104
Attn: (Pathology or Genomics Personnel Here)
North Grafton, MA 01536
CPathGenSR@tufts.edu