Molecular Helminthology Laboratory Schistosome Publications

  1. Da'dara AA, Bhardwaj R, Ali YB, Skelly PJ. Schistosome tegumental ecto-apyrase (SmATPDase1) degrades exogenous pro-inflammatory and pro-thrombotic nucleotides. PeerJ. 2014 Mar 18;2:e316. doi: 10.7717/peerj.316.
  2. Skelly PJ. The use of imaging to detect schistosomes and diagnose schistosomiasis. Parasite Immunol. 2013 Sep-Oct;35(9-10):295-301. doi: 10.1111/pim.12040.
  3. Da'dara AA, Faghiri Z, Krautz-Peterson G, Bhardwaj R, Skelly. 2013. PJ.Schistosome Na,K-ATPase as a therapeutic target. Trans R Soc Trop Med Hyg. 107:74-82. PMID: 23222953
  4. Da'dara A, Krautz-Peterson G, Faghiri Z, Skelly PJ. 2012. Metabolite movement across the schistosome surface. J Helminthol. 86:141-7. PMID: 22365312
  5. Swain MT, Larkin DM, Caffrey CR, Davies SJ, Loukas A, Skelly PJ, Hoffmann KF. 2011. Schistosoma comparative genomics: integrating genome structure, parasite biology and anthelmintic discovery.Trends Parasitol. 27:555-64. PMID: 22024648
  6. Bhardwaj R, Krautz-Peterson G, Da'dara A, Tzipori S, Skelly 2011 PJ.Tegumental phosphodiesterase SmNPP-5 is a virulence factor for schistosomes. Infect Immun. 79:4276-84. PMID: 21825060
  7. Bhardwaj R, Krautz-Peterson G, Skelly PJ. 2011. Using RNA Interference in Schistosoma mansoni. Methods Mol Biol. 764:223-39. PMID: 21748644
  8. Da'dara A, Skelly PJ. 2011. Manipulation of vascular function by blood flukes? Blood Rev. 2011 25:175-9. PMID: 21543145
  9. Bhardwaj R, Skelly PJ. 2011. Characterization of schistosome tegumental alkaline phosphatase (SmAP). PLoS Negl Trop Dis. 2011 Apr 5;5(4):e1011. PMID:21483710
  10. Sepulveda J, Tremblay JM, Skelly PJ, Shoemaker CB. 2010. Schistosoma mansoni host-exposed surface antigens characterized by sera and recombinant antibodies from schistosomiasis resistant rats. Int. J. Parasitol. 40:1407-17.
  11. Krautz-Peterson G, Simoes M, Faghiri Z, Ndegwa D, Oliveira G, Shoemaker CB, Skelly PJ. 2010. Suppressing glucose transporter gene expression in schistosomes impairs parasite feeding and decreases survival in the mammalian host. Plos Pathogens. 6:e1000932.
  12. Schroeder, H, Skelly, PJ Zipfel, PF, Losson, B, Vanderplasschen, A. Subversion of complement by hematophagous parasites. Dev. Comp. Imm., 2009; 33: 5–13.
  13. Skelly, PJ. Fighting Killer Worms. Scientific American, May 2008,Vol 298, No.5, 72-77
  14. Krautz-Peterson, G, Skelly PJ. Schistosome asparaginyl endopeptidase (legumain) is not essential for cathepsin B1 activation in vivo. Mol Biochem Parasitol 2008; 2008 May; 159(1):54-8.
  15. Skelly, PJ. Sex and the Single Schistosome. Natural History, June 2008, Vol 117, No.5, 22-27
  16. Sepulveda, J, Shoemaker, CB. 2008. Design and testing of PCR primers for the construction of scFv libraries representing the immunoglobulin repertoire of rats. J Immunol Methods. 332:92-102.
  17. Krautz-Peterson, G, Skelly, PJ. Schistosoma mansoni: The dicer gene and its expression. Exp Parasitol. 2007; Jan; 118(1):122-8.
  18. Ndegwa D, Krautz-Peterson, G, Skelly, PJ. Protocols for gene silencing in schistosomes. Exp Parasitol, 2007; 117(3), 284-291.
  19. Krautz-Peterson, G, Camargo, S, Huggel, K, Verrey, F, Shoemaker, CB, Skelly PJ. 2007. Amino acid transport in schistosomes: characterization of the permease heavy chain SPRM1hc. J. Biol. Chem. 282:21767-75.
  20. Krautz-Peterson, G, Radwanska, M, Ndegwa, D, Shoemaker, CB, Skelly, PJ. 2007. Optimizing gene suppression in schistosomes using RNA interference. Mol. Biochem. Parasitol. 153:194-202.
  21. Van Hellemond JJ, Retra K, Brouwers JFHM, van Balkom BWM, Yazdanbakhsh M, Shoemaker CB, Tielens, AGM. 2006. Functions of the tegument of schistosomes: clues from the proteome and lipidome. Int. J. Parasitol. 31:691-699.
  22. Skelly, PJ. Fighting killer worms. Scientific American, 2008; 298 (5): 94-99.
  23. Krautz-Peterson, G; Skelly, PJ. Schistosome asparaginyl > endopeptidase (legumain) is not essential for cathepsin B1 activation in vivo. Mol Biochem Parasitol, 2008; 159 (1): 54-58.
  24. Skelly, PJ. Sex and the single schistosome. Natural History, 2008; 117 (5):22-27.
  25. Krautz-Peterson, G, Skelly, PJ. Schistosoma mansoni: The Dicer gene and its expression. Exp Parasitol, 2008; 118 (1): 122-128.
  26. Ndegwa D, Krautz-Peterson, G, and Skelly, PJ. Protocols for gene silencing in schistosomes. Exp Parasitol, 2007; 117(3), 284-291.
  27. Skelly PJ. 2005. The surface of schistosomes within the vertebrate host. Chapter 6 in "Schistosomiasis" Vol 10 in the World Class Parasite series. Secor, W. E., Colley, D. G. (Eds.). Kluwer Press.
  28. Skelly PJ. 2004. Intravascular schistosomes and complement. Trends Parasitol. 20, 370-4.
  29. Dalton JP, Skelly PJ and Halton DW. 2004. The role of the tegument and gut in the uptake of nutrients by parasitic platyhelminths. Can J Zool, 82, 211-232.
  30. Skelly PJ, Da'dara AA and Harn DA. 2003. Suppression of cathepsin B expression in Schistosoma mansoniby RNA interference. Int J Parasitol, 33, 363-9.
  31. Boyle, JP, Wu, X, Shoemaker, CB, Yoshino, TP. 2003. Using RNA interference to manipulate endogenous gene expression in Schistosoma mansonisporocysts. Mol. Biochem. Parasitol. 128:205-15.
  32. Da'dara AA Skelly PJ, Walker CM and Harn DA. 2003. A DNA-priming protein-boosting regimen significantly improves type 2 immune responses but not protective immunity to Schistosoma mansoniinfection. Parasite Immunol, 25, 429-37.
  33. Da'dara AA, Skelly PJ, Fatakdawala M, Visocatti S, Eriksson E and Harn DA. 2002. Comparative efficacy of the Schistosoma mansoninucleic acid vaccine, Sm23, following microseeding or gene gun delivery. Parasite Immunol, 24, 179-87.
  34. Da'dara A, Tsai MH, Tao LF, Marx KA, Shoemaker CB, Harn DA, Skelly PJ. 2001. Schistosoma mansoni: molecular characterization of a tegumental Ca-ATPase (SMA3). Exp Parasitol. 98:215-22.
  35. Skelly, PJ and Shoemaker, CB. 2001. Schistosoma mansoniproteases Sm31 (cathepsin B) and Sm32 (legumain) are expressed in the caecum and protonephridia of cercariae. J. Parasitol. 87:1218-21.
  36. El Ridi, R, Shoemaker, CB, Farouk, F, El Sherif, NH, and Afifi, A. 2001. Human T and B cell responses to Schistosoma mansonirecombinant glyceraldehyde 3-phosphate dehydrogenase correlate with resistance to reinfection with S. mansoni or S. haematobium after chemotherapy. Infect. Immun. 69:237-44.
  37. Skelly, PJ and Shoemaker, CB. 2001. The Schistosoma mansonihost interactive tegument forms from vesicle eruptions of a cyton network. Parasitology. 122:67-73.
  38. Skelly, PJ, Dougan, PM, Maule, A, Day, TA and Shoemaker, CB. 2001. Cloning and characterization of a muscle isoform of a Sodium Potassium ATPase alpha subunit (SNaK1) from Schistosoma mansoni. Parasitology. 123:277-84.
  39. Skelly, PJ and Shoemaker, CB. 2000. Induction cues for tegument formation during the transformation of Schistosoma mansonicercariae. Int. J. Parasitol. 24:625-31.
  40. Skelly, PJ, Pfeiffer, R Verrey, F, and Shoemaker, CB. 1999. SPRM1lc a heterodimeric amino acid permease light chain of the human parasitic platyhelminth, Schistosoma mansoni. Parasitology. 119:569-576.
  41. Brouwers, JFHM, Skelly, PJ, Van Golde, LMG and Tielens, AGM. 1999. Studies on phospholipid turnover argue against sloughing of tegumental membranes in adult Schistosoma mansoni. Parasitology, 119, 287-94.
  42. El Ridi, R, Farouk, F, Sherif, M, Al-Sherminy, M, Osman, A, El Gengehi, N and Shoemaker, CB. 1998. T and B cell reactivity to a 42 kDa protein is associated with human resistance to both schistosomiasis mansoni and haematobium. J. Inf. Dis. 177:1364-72.
  43. Skelly, PJ, Tielens, AGM and Shoemaker, CB. 1998. Glucose transport and metabolism in mammalian stage schistosomes. Parasitol. Today. 14:402-406.
  44. Mastroberardino, L., Spindler, B., Pfeiffer, R., Skelly, PJ, Shoemaker, C and Verrey, F. 1998 Amino acid transport by heterodimers of 4F2/CD98 and members of a permease family. Nature, 395, 288-92.
  45. Davies, SJ, Shoemaker, CB and Pearce, EJ. 1998. A divergent member of the transforming growth factor b receptor family from Schistosoma mansoniis expressed on the parasite surface membrane. J. Biol. Chem. 273:11234-40.
  46. Skelly, PJ and Shoemaker, CB. 1996. Rapid Appearance and Asymmetric Distribution of Glucose Transporter, SGTP4, at the Apical Surface of Intramammalian Stage Schistosoma mansoni. Proc. Natl. Acad. Sci. USA.93:3642-3646.
  47. Jiang, J, Skelly, PJ, Shoemaker, CB and Caulfield, JP. 1996. Schistosoma mansoni: The glucose transport protein SGTP4 is present in the tegumental multilamellar bodies, discoid bodies, and the surface lipid bilayers. Exp. Parasitol. 82:201-210.
  48. Armstrong, RL, Wilson, JE and Shoemaker, CB. 1996. Purification and characterization of the hexokinase from Schistosoma mansoni, expressed in E. coli. Prot. Expr. and Purif. 8:374-380.
  49. Ramachandran, H, Skelly, PJ and Shoemaker, CB. 1996. The Schistosoma mansoniepidermal growth factor receptor homologue, SER, has tyrosine kinase activity and is localized in adult muscle. Mol. Biol. Parasitol. 83:1-10.
  50. Shoemaker CB, Reynolds S, Wei G, Tielens, AGM, Harn D. 1995. Schistosoma mansonihexokinase: cDNA cloning and immunogenicity studies. Exp. Parasitol. 80:36-45.
  51. Zhong, C, Skelly, PJ, Leaffer, D, Cohn, RL, Caulfield, JP and Shoemaker, CB. 1995. Immunolocalization of a Schistosoma mansonifacilitated glucose transporter to the basal, but not the apical membranes of the surface syncytium. Parasitology. 110:383-394.
  52. Skelly PJ and Shoemaker, CB. 1995. A molecular genetic study of the variations in metabolic function during schistosome development. Mem. do Inst. Osw. Cruz. 90:281-284.
  53. Laclette, JP, Skelly, PJ, Merchant, MT, Shoemaker, CB. 1995. Aldehyde fixation dramatically alters the immunolocalization pattern of paramyosin in platyhelminth parasites. Exp. Parasitol. 81:140-143.
  54. Skelly PJ, Kim, JW, Cunningham J, Shoemaker, CB. 1994. Cloning, characterization and functional expression of cDNAs encoding glucose transporter proteins from the human parasite, Schistosoma mansoni. J. Biol. Chem. 269:4247-4253.
  55. Bosch IB, Wang XZ, Shoemaker CB. 1994. Two Schistosoma mansonicDNAs encoding ATP cassette (ABC) family proteins. Mol. Biochem. Parasitol. 65:351-356.
  56. Skelly PJ, Secor WE, Reis MG, Ramos EA, Carmo TM, Reis EA and Harn DA. 1994. Failure of schistosomiasis to significantly decrease testosterone levels in Brazilian men. Am J Trop Med Hyg, 51(1), 40-4.
  57. Shoemaker, CB. 1994. Schistosoma mansoniphosphagen kinase gene contains two closely apposed transcription initiation sites and arose from a fused gene duplication. Mol. Biochem. Parasitol. 68:319-322.
  58. Tielens, AGM, van den Heuvel, JM, van Mazijk, HJ, Wilson, J and Shoemaker, CB. 1994. The 50-kDA, glucose-6-phosphate sensitive hexokinase of Schistosoma mansoni, enzymatic properties and amino- acid sequence analysis. J. Biol. Chem. 269:24736-24741.
  59. dos Reis MG, Davis RE, Singh H, Skelly PJ, Shoemaker CB. 1993. Characterization of the Schistosoma mansonigene encoding the glycolytic enzyme, triose phosphate isomerase. Mol. Biochem. Parasitol. 59:235-242.
  60. Skelly PJ, Stein L, Shoemaker CB. 1993. Expression of Schistosoma mansoniGenes Involved in Anaerobic and Oxidative Glucose Metabolism During the Cercarial to Adult Transformation. Mol. Biochem. Parasitol. 60:93-104.
  61. Shoemaker CB, Gross A, Gebremichael A, Harn D. 1992. cDNA cloning and functional expression of the Schistosoma mansoniprotective antigen, triose phosphate isomerase. Proc. Natl. Acad. Sci. USA. 89:1842-1846.
  62. Shoemaker CB., Ramachandran H, Landa A, Reis M, Stein L. 1992. Alternate splicing of the Schistosoma mansonigene encoding a homologue of epidermal growth factor receptor. Mol. Biochem. Parasitol. 53:17-32.
  63. Reynolds SR, Shoemaker CB, Harn DA. 1992. T and B cell epitope mapping of SM23, an integral membrane protein of Schistosoma mansoni. J. Immunol. 149:3995-4001.
  64. Laclette JP, Landa A, Arcos L, Willms K, Davis E, Shoemaker CB. 1991. Paramyosin is the Schistosoma mansoni(trematoda) homologue of antigen B from Taenia solium(cestoda). Mol. Biochem. Parasitol 44:287-296