Department of Biomedical Sciences
Developmental and Molecular Biology
Personal URL: http://www.tufts.edu/sackler/cmdb/sarkar-lab.htm
Dept. URL: http://www.tufts.edu/med/anatomy/
PhD - Northwestern University - 1961
MS - University of Calcutta, India - 1956
BS (Hons.) - University of Calcutta, India - 1953
Monalisa Mukherjea, M.S., Graduate Student
Tathagata Chaudhuri, M.S., Graduate Student
Subhradip Mukhopadhyay, M.S., Graduate Student
General Research Interest
My laboratory has been engaged in studies on the biochemistry and molecular biology of vertebrate striated muscle since 1969. The research, continuously funded by NIH for the period of 1969-2003, spans various aspects of muscle biology (see also publications).
Research Sponsor Interest
- Privately Funded Research
- Federally Funded Research
Selected Research Projects
Current studies include the evolution of vertebrate striated muscle genes, the regulation of gene expression in muscle cell during development, structure of human muscle genes and their expression in normal and pathological conditions, and mechanism of Ca-regulated muscle contraction by using mutant muscle proteins.
Research and Clinical Interests
Genetic aspects of infantile dilated cardiomyopathy in Portuguese Water Dogs
Anti-angiogenesis activity of thin filament muscle proteins
Structure and function of troponin complex
Evolution of multigene families of contractile proteins
Mechanism of Ca regulation of muscle contraction
Role of troponin T in thin filament function
Coiled coil interactions of muscle proteins
Major Specialized Equipment Items Available
PCRl; Fluorescence Spectrophotometer; Hybridization Chamber for genomic and cDNA hybridization; other standard equipment for DNA, RNA and protein work
Stefancsik, R. and Sarkar, S. 2003. Relationship between the DNA binding
domains of SMAD and NFI/CT F transcription factors defines a new superfamily
of genes. DNA Sequence 14:233-239.
Sachdev, S., Raychowdhury, M.K. and Sarkar, S. 2003. Human fast skeletal
myosin light chain 2 cDNA: Isolation, tissue specific expression of the
single copy gene, comparative sequence analysis of isoforms and evolutionary
relationships. DNA Sequence 14: 339-350.
Stefancsik, R., Randall, J.D. and Sarkar, S. 2003. Structure and sequence
of human fast skeletal troponin T (TNNT3) gene: Insight into the evolution
of the gene and origin of the developmentally regulated isoforms.
Comparative and Functional Genomics 4: 609-625.
Alroy, J., Rush, J.E., Freeman, L. , Kumar, M.S.A., Karuri, A., Chase, K.,
Sarkar, S. 2000. Inherited Infantile Dilated Cardiomyopathy in Dogs:
Genetic, Clinical, Biochemical and Morphological Findings. Am. J.
Med. Genetics 95:57-66. Web page of publications: http://www.cos.com